TMIC-81. INCREASED LEVEL OF IGFBP2 PROMOTES TUMOR METASTASIS IN SHH MEDULLOBLASTOMA

Abstract Medulloblastoma (MB) is the most common pediatric brain malignancy. MB comprises 5 major subgroups known as WNT, SHH p53wt, p53mut, Group 3 and 4. Among four group dominant molecular subgroup in infants adults. These tumors are proposed to arise from cerebellar granule neuron precursors (CGNPs), whose developmental expansion requires signaling neighboring Purkinje neurons. Previous reports suggest that features a unique tumor microenvironment compared with other groups. To better understand how cells interact Tumor Microenvironment, we performed cytokine array analysis of culture media Patient cells, spontaneous mouse cell lines. Further, confirmed these results using ELISA, Western blot, immunofluorescence human lines, Smo/A1 primary PZp53Med In continuation observation IGFBP2 expression various types single analysis, analyzed presence astrocytes Immunohistochemistry. Our data showed increased levels produced by lines others. We role growth metastasis. knock-down stable phenotypic changes including reduced proliferation, migration EMT. Further western blot KD EMT markers also activation STAT3. preliminary vitro exerts it metastasis-promoting regulating marker proteins matrix remodeling proteins. functional studies MB, may regulate STAT3-mediated program metastasize. findings provide strong rationale for further pursuing promotes medulloblastoma vivo.